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To challenge our RIT a second cohort of animals bearing larger tumors (470 ± 121 mm³) received the same treatment regimen. We then investigated the impact of 177LuLu-DUNP19 radioimmunotherapy (RIT) on tumor volume and overall survival in mice bearing HuO9 osteosarcoma tumors. This co-localization suggests cellular internalization of the antibody after binding with LRRC15 on the plasma membrane of both cancer and stromal cells. Next, we investigated the subcellular localization of DUNP19 in human osteosarcoma (SAOS2 and HuO9), and breast cancer (HCC1954) tumor models following systemic administration. Tumor models with LRRC15+ cancer cells included K7M2LRRC15+, HuO9 and SAOS2 osteosarcomas (OS) and U118MG glioblastoma (GBM).

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  • Mice were euthanized when subcutaneous tumors exceeded 1000 mm³ (15 mm in diameter), when ulceration or bleeding sores developed on the tumor, or when significant overall health decline was observed, including a body weight loss exceeding 20% of baseline. newlineCells were fixed with 3.7% paraformaldehyde for 15 min at RT before washing and mounting cells with Vectashield mounting media with DAPI (H ) onto slides for imaging with a Leica TCS SP8 Digital Microscope.
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  • In osteosarcoma models in which tumor cells directly expressed LRRC15, 177LuLu-DUNP19 treatment significantly inhibited tumor growth and extended survival in both subcutaneous xenografts and translationally relevant orthotopic models.
  • Imaging by SPECT at 72 h post-177LuLu-DUNP19 administration revealed LRRC15-specific accumulation of activity at the tumor site (Fig. 3d, e).
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  • HuO9 (LRRC15+ cancer cells) and HCC1954 (LRRC15- cancer cells/LRRC15+ stroma) tumors harvested at 72 h post- i.v.

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Continuous progression-free survival was sustained in treated animals throughout the study duration, in contrast to untreated animals which exhibited a median survival of 47.5 days (p 3h). We evaluated the impact of sequential and fractionated dosing on tumor growth and survival. Assessment of tumor volumes demonstrated that repeated cycles of LRRC15-RIT effectively inhibit tumor growth (g). 177LuLu-DUNP19 (at day 0, 32, and 75), resulting in a total administered activity of 50 MBq, or no treatment (control; black). Kaplan–Meier survival analysis revealed that 177LuLu-DUNP19 extended survival, with the impact varying based on the timing of intervention (c).

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